Senior Research Associate, Stem Cell Engineering at Colossal Biosciences

Colossal Biosciences is seeking a skilled and motivated stem cell engineer to support our efforts in engineering embryonic and pluripotent cell systems for non-model species. This role will work closely with scientists across our mammalian de-extinction and conservation programs to optimize and apply protocols in embryonic and induced pluripotent stem cell culture reprogramming and 3D cell systems.The Senior Research Associate will contribute to experimental planning execution and documentation and play a key role in advancing foundational stem cell platforms for use in cellular engineering embryo support and translational biology across marsupial role involves close collaboration with multiple internal teams and requires excellent communication adaptability and a strong team-oriented mindset.This position is based on-site in our Dallas TX location. Relocation assistance is available.Duties and ResponsibilitiesContribute to the design development and execution of novel experiments techniques and protocols under the guidance of scientists with growing autonomy in protocol optimization and troubleshooting.Perform and support 2D and 3D cell culture of primary immortalized ESC and iPSC lines with high technical accuracy.Conduct and troubleshoot FACS Western blotting qPCR immunostaining transfection and microscopy-based assays.Support stem cell reprogramming workflows including those for non-model species with adaptability and a willingness to learn species-specific protocols.Maintain and optimize 3D culture systems including spheroid and organoid models.Characterize and perform quality control of marsupial stem cell cultures with training provided where needed.Document experimental work thoroughly and contribute to reports presentations and publications.Maintain accurate up-to-date digital lab records and follow team SOPs and quality standards.Support live cell maintenance including occasional weekend work depending on feeding or culture requirements.Skills and AbilitiesRequiredExperience working with primary embryonic and iPSC cultures including maintenance and expansion techniques.Proficiency in core molecular biology techniques including qPCR immunohistochemistry colony picking and stem cell passaging workflows.Familiarity with stem cell reprogramming technologies particularly iPSC generation and validation.Experience using electronic lab notebooks (ELNs) and inventory management systems (e.g. Benchling) for data tracking and reagent management.PreferredExperience with RNA extraction RNA handling and protein quantification assays (e.g. ELISA and Western blot).Experience with cell line derivation propagation and transfection methodologies especially when following established protocols.Familiarity with sequence analysis software such as SnapGene or Geneious.Education and ExperienceBachelors or Masters degree in biological sciences molecular and cell biology bioengineering biochemistry developmental biology or a related field.Prior experience as a Research Associate or Technician in an academic or biotechnology industry setting is a plus.Experience with non-model systems or comparative biology is welcomed but not required.What Colossal Offers Full-Time Employees:Medical dental and vision coverageExcellent paid time off and company holidays so you can rest and rechargeFlexible spending accounts (FSA)Company matched 401k retirement planPaid parental leave at 100% salary for up to 12 weeksEducation reimbursementAn opportunity to help us return the Earth to a healthier stateThe team at Colossal is committed colorful passionate intelligent collaborative creative experienced scientific and world-class. That sounds like a lot to fit into one building. Thats why we are spread out in several locations that include laboratories test facilities academic institutions libraries and offices.Through it all however we maintain a culture of positivity hope happiness and belief that were doing something big. Something Colossal.Colossal is proud to be an equal employment opportunity workplace. We welcome people from all backgrounds as well as celebrate and support diversity. We are committed to providing an inclusive environment of mutual respect that is free from discrimination and harassment so everyone can thrive.EEO is the LawSearch Firm Representatives: Colossal does not accept unsolicited assistance from search firms. No fees will be paid for a hired candidate where no pre-existing agreement is in place. We handle most recruitment in-house.Colossal is proud to be named a Best Place to Work by BuiltIn for 3 years in a row across multiple categories inand 2025:#1 on the 50 Best Startups to Work For in Dallas in 2023!100 Best Midsize Places to Work in Dallas in 2024 & 2025100 Best Places to Work in Dallas TX (All Size Employers) in 2024 & 2025 Built In 2025 Award ListsGreat Place to Work Certification PageMost Loved Workplace Certification Page - Proud to be certified as a most loved workplace including: most loved CEO most loved workplaces for LGBTQ most loved workplaces for women and most loved workplaces for young professionals.Newsweeks Most Loved Workplaces 2024 Award List - Excited to be amongst the winners of Newsweeks 2024 Most Loved Workplaces! Required Experience:Senior IC Key Skills Laboratory Experience,Immunoassays,Mammalian Cell Culture,PCR,Biochemistry,Assays,Cell Biology,Research Experience,Cell Culture,Molecular Biology,Flow Cytometry,Western Blot Employment Type : Full Time Experience: years Vacancy: 1