Staff Scientist, Biochemistry, Brown at Vanderbilt University

DescriptionAbout the Work Unit:The Brown Laboratory focuses on understanding defects in macromolecular protein assembly that underlie human disease. Specifically using structural biology and complementary techniques we seek to determine the structural basis of protein assembly that is critical for proper mitochondrial physiology; defects in which cause severe diseases ranging from anemia to neurodegeneration. The laboratory also employs a wide range of biochemical biophysical and cellular techniques to complement our structural work including mass spectrometry fluorescence-based enzyme assays quantitative binding assays and mammalian cell culture.Duties and Responsibilities:The individual is expected to support research activities that include but are not limited to: molecular cloning protein expression and purification biochemical and biophysical assay development cellular analysis and general laboratory operations support.The ideal candidate would have prior laboratory experience working in biochemistry or related fields. The ability to operate independently as well as part of a team is critical.Preferred experience and skills:Molecular cloning to prepare various constructs and variants of the proteins of interest with suitable vectors for recombinant protein expression and various assaysMaintenance of mammalian cell linesDevelop and conduct cell-based assays such as fluorescence-activated cell sorting (FACS) to study the function of a large number of variants of the proteins of interestRecombinant protein expressionProtein purification using centrifugation affinity chromatography ion-exchange and size-exclusion chromatographyBiochemical and biophysical assay development to measure protein activity protein-protein and protein-small molecule interactionsGeneral duties in laboratory research and development:Provide technical support for different projects in the labParticipate in laboratory meetings and planning sessionsCollect record organize and verify the accuracy of experimental dataPerform data analysis and prepare charts graphs and reports for data presentationSupervisory Relationships:This position does not have supervisory responsibility.Education and Certifications: Profile of an Ideal CandidatePh.D. in physical or biological sciences is required.Qualifications:At least three years of post-graduate experience required.Laboratory experience in proteomics biochemistry cell biology and molecular biology is requiredExperiencewithorganizationinventoryandrecord-keepingispreferredSkills:Strong organizational skillsStrong written and verbal communication skillsExcellent team playerRequired Experience:Staff IC Key Skills Laboratory Experience,Bioinformatics,Biochemistry,Utility Locating,Assays,Research Experience,Next Generation Sequencing,Sensors,Signal Processing,Matlab,Research & Development,Molecular Biology Employment Type : Full-Time Experience: years Vacancy: 1